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inqaba biotec™ Product Portfolio - ‘one stop shop’ for life sciences
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Sanger DNA Sequencing Menu Home Services Products About Us Southern Africa East Africa West Africa Blog Contact Us Oligos Login Events View Events Submit EventHodgepodgeRequest Home Services Sanger Sequencing Sanger Sequencing DNA sequencing is rhadamanthine increasingly important in all disciplines of molecular biology, as well as in unromantic fields such as diagnostics or forensic research. The speed at which one obtains the results is therefore a hair-trigger factor in the efficiency of genomic research and diagnostic work. In 2010, inqaba biotec™ upgraded the sequencing laboratory by the vanquishment of an ABI 3500XL genetic analyser. The vanquishment has decreased our turnaround time and improved the quality of our reads. The market response was so overwhelming that we uninventive a second ABI 3500XL genetic analyser soon after. At present, we produce increasingly than 10 000 reads per month. Sanger Service Exceptional, efficient and energetic technicians perform a variety of molecular techniques such as PCR and Sanger Sequencing reactions at the highest level of precision and accuracy. inqaba biotec™ offers tailor-made sequencing services: single reaction to whole genome; SNP discovery to validation projects. We offer a wide range of services including SIMPLE LANE reactions (giving you maximum control) to our FULL SERVICE. We endeavour to alimony our service as flexible as possible to unbend your sequencing requirements. Our Sanger Sequencing Department provides the pursuit services to suite your individual needs: Simple Lane – A sequence reaction is submitted. All sequencing reactions should be submitted protected from light. Basic Service – A purified PCR product/high quality plasmid DNA and primers are provided to inqaba biotec™ at the correct concentrations. inqaba biotec™ performs sequencing reaction, purification and injection on the ABI 3500XL Genetic Analyzer. Full Service – Unpurified PCR product/plasmid is submitted to inqaba biotec™ . inqaba biotec™ performs purification and quantification of the template, sequencing reaction, purification and injection on the ABI 3500XL Genetic Analyzer. Standard Sequencing parameters: ABI 3500XL Genetic Analyzer, POP7™, BrilliantDye™ Terminator v3.1. Please take note that in order to streamline all processes in the laboratory we request that you provide samples, PCR products, primers and plasmids with the pursuit parameters: Primers – minimum 10μl with a concentration of 10μM + 1μl for every reaction required. PCR products – minimum 15μl Plasmids – minimum 12μl gDNA – minimum of 15μl with a minimum concentration of 30ng/μl + 1μl for every reaction required. Click here for increasingly sequencing details. Want to send us samples, well-constructed our Sequencing_Form_2018. Require hodgepodge of your samples? Click here. Contact Details Southern Africa Inqaba Biotechnical Industries (Pty) Ltd. PO Box 14356, Hatfield 0028 Pretoria, South Africa Tel: +27 12 343 5829 Fax: +27 86 677 8409 E-mail: East Africa inqaba biotec East Africa Ltd. PO Box 1846 Nairobi 00606, Kenya Tel: +254 735 370 693 Fax: +27 86 677 8409 E-mail: West Africa inqaba biotec West Africa Ltd. PMB 5320, Oyo Road, Ibadan 200001 Oyo State, Nigeria Tel: +‎234 805 882 7272 Fax: +27 86 677 8409 E-mail: Copyright © Inqaba Biotechnical Industries (Pty) Ltd 2018 Home Services Products About Us Contact Us